4NFX
Structure and atypical hydrolysis mechanism of the Nudix hydrolase Orf153 (YmfB) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-05-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96405 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.960, 70.521, 145.031 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 33.560 - 2.690 |
| R-factor | 0.23617 |
| Rwork | 0.233 |
| R-free | 0.28807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 40006 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 283 | 0.1M sodium citrate buffer pH 4.0, 3.5%(w/v) PEG4000, 64mM magnesium formate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
