4NFV
Previously de-ionized HEW lysozyme batch crystallized in 1.1 M MnCl2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE D41A |
Synchrotron site | LURE |
Beamline | D41A |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2000-11-30 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.607, 79.607, 37.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.445 - 1.630 |
R-factor | 0.15111 |
Rwork | 0.148 |
R-free | 0.17919 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.020 |
RMSD bond angle | 1.778 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.445 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 215410 | |
<I/σ(I)> | 23.8 | 4.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.7 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | Previously de-ionized lysozyme, no buffer added, 1.1 M MnCl2, pH 4.5, Batch crystallization, temperature 293K |