4NFU
Structure of the central plant immunity signaling node EDS1 in complex with its interaction partner SAG101
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0399, 1.0404, 1.0313, 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.586, 113.643, 125.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.278 - 2.210 |
R-factor | 0.1768 |
Rwork | 0.176 |
R-free | 0.19550 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.623 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHARP (INTEGRATED INTO AUTO-RICKSHAW (EMBL-Hamburg)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.054 | 0.972 |
Number of reflections | 78935 | |
<I/σ(I)> | 21.6 | 2 |
Completeness [%] | 97.3 | 79.1 |
Redundancy | 7.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 5% w/v PEG4000, 5% 2-propanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |