4NFO
Crystal Structure Analysis of the 16mer GCAGACUUAAGUCUGC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.148 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.980, 43.080, 48.760 |
Unit cell angles | 90.00, 120.98, 90.00 |
Refinement procedure
Resolution | 35.810 - 1.960 |
R-factor | 0.21839 |
Rwork | 0.217 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.584 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.810 | 2.010 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.020 | 0.310 |
Number of reflections | 9115 | |
<I/σ(I)> | 17.06 | 2.19 |
Completeness [%] | 93.4 | 96.7 |
Redundancy | 2 | 1.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294.15 | 10% MPD(v/v)(Drop), 35% MPD(v/v)(reservoir), 40mM Na-cacodylate trihydrate (pH 7), 12mM spermine tetrahydrochloride, 80mM SrCl2, 20mM MgCl2 hexahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |