4NF9
Structure of the Knl1/Nsl1 complex
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0006 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.710, 71.630, 177.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.310 - 2.800 |
| R-factor | 0.20989 |
| Rwork | 0.207 |
| R-free | 0.26502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.310 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.087 | |
| Number of reflections | 36465 | |
| <I/σ(I)> | 14.09 | |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 16-20 % (v/v) PEG 3350, 220 mM sodium citrate and 100 mM MES (pH 6 or 6.5) and 100 mM Hepes (pH 7), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
