4NEI
Alg17c PL17 Family Alginate Lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.530, 126.351, 87.631 |
| Unit cell angles | 90.00, 111.51, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| R-factor | 0.16957 |
| Rwork | 0.168 |
| R-free | 0.19228 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 144904 | |
| Completeness [%] | 98.3 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 10% PEG 6000, 5% MPD, 100 mM Tris, pH=8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
