4NE6
Human MHF1-MHF2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.734, 40.819, 59.480 |
| Unit cell angles | 91.90, 105.55, 95.47 |
Refinement procedure
| Resolution | 37.310 - 2.100 |
| R-factor | 0.2321 |
| Rwork | 0.230 |
| R-free | 0.26180 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.310 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 20776 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Micro-batch under oil | 300 | Micro-batch under oil, temperature 300K |
