4NDN
Structural insights of MAT enzymes: MATa2b complexed with SAM and PPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.140, 122.178, 298.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.780 - 2.340 |
R-factor | 0.2126 |
Rwork | 0.211 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
High resolution limit [Å] | 2.340 | 5.060 | 2.350 |
Rmerge | 0.101 | 0.045 | 0.704 |
Number of reflections | 109240 | ||
<I/σ(I)> | 6.9 | ||
Completeness [%] | 98.0 | 99.6 | 83.2 |
Redundancy | 7.5 | 7.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 100mM MES/Imidazole buffer 10% Ethylene glycol 20% PEG 8K, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |