4NDJ

Crystal Structure of a computational designed engrailed homeodomain variant fused with YFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	150
Detector technology	PIXEL
Collection date	2009-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	P 42 2 2
Unit cell lengths	104.420, 104.420, 80.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.961 - 1.850
R-factor	0.1745
Rwork	0.173
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.191
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
High resolution limit [Å]	1.850
Number of reflections	39983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.8 M monosodium phosphate, 1.2 M dipotassium phosphate, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K