4NCW
foldon domain wild type C-conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.710, 28.100, 55.990 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.16984 |
| Rwork | 0.168 |
| R-free | 0.19299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.071 | 0.289 |
| Number of reflections | 17100 | |
| <I/σ(I)> | 9.3 | 3.6 |
| Completeness [%] | 94.4 | 92.5 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Ammoniumsulfate, 30% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
