4NCK
Crystal Structure of Pyrococcus furiosis Rad50 R797G mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.669, 68.065, 202.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.245 - 1.990 |
R-factor | 0.1831 |
Rwork | 0.181 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.274 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_1233) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 | |
High resolution limit [Å] | 1.990 | 8.930 | 1.990 |
Rmerge | 0.036 | 0.017 | 0.784 |
Number of reflections | 89299 | ||
<I/σ(I)> | 16.22 | 47.41 | 1.4 |
Completeness [%] | 98.8 | 87.5 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 100 mM Bicine, 6-14% PEG 20000, 2 mM Dioxane, pH 8.6, vapor diffusion, sitting drop, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 100 mM Bicine, 6-14% PEG 20000, 2 mM Dioxane, pH 8.6, vapor diffusion, sitting drop, temperature 293K |