4NCD
Crystal Structure of Class 5 Fimbriae Chaperone CfaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.606, 28.582, 90.716 |
| Unit cell angles | 90.00, 118.89, 90.00 |
Refinement procedure
| Resolution | 39.715 - 2.037 |
| R-factor | 0.2213 |
| Rwork | 0.217 |
| R-free | 0.25640 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.364 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 15399 | |
| Completeness [%] | 97.4 | 72.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.3 | 293 | 22% PEG3350, 0.2 M NaCl, 0.1 M MES pH 5.3, EVAPORATION, temperature 293K |
