4NCC
Neutralizing antibody to murine norovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-01 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.590, 135.810, 83.310 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 42.490 - 2.490 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.490 |
| High resolution limit [Å] | 2.490 |
| Number of reflections | 20909 |
| Completeness [%] | 63.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 298 | Protein concentration 9.7 mg/ml in 50mM Tris. Reservoir 18% PEG 8000 in 90 mM Tris, pH 8.5. Drop was 5 microliters of reservoir and 5 of protein., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
