4NC6

Tbc domain of human rab gtpase-activating protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	43.150, 51.390, 132.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 1.800
R-factor	0.16244
Rwork	0.160
R-free	0.21492
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hzj
RMSD bond length	0.018
RMSD bond angle	1.811
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.067	0.685
Number of reflections	27834
<I/σ(I)>	23.1	2.45
Completeness [%]	99.2	91.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	27%(W/V) PEG2000 MME, 100MM NA ACETATE , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K