4NBO

Human steroid receptor RNA activator protein carboxy-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	85.901, 85.901, 100.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.489 - 2.807
R-factor	0.2468
Rwork	0.245
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yru
RMSD bond length	0.004
RMSD bond angle	0.752
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	9695
Completeness [%]	99.9	100
Redundancy	7.4	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	1.4-2.0 Malonate(Na+), 0.1 M Mops as precipitant, stabilized in 2.4 M Malonate, 0.1 M MOPS pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K