4NBM

Crystal structure of UVB photoreceptor UVR8 and light-induced structural changes at 180K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-05
Detector	RAYONIX MX-300
Spacegroup name	C 1 2 1
Unit cell lengths	113.310, 76.828, 191.253
Unit cell angles	90.00, 96.75, 90.00

Refinement procedure

Resolution	29.860 - 1.610
R-factor	0.151
Rwork	0.150
R-free	0.18200
Structure solution method	AND DIFFERENCE FOURIER FOR LIIGHT-INDUCED STRUCTURAL CHANGES
RMSD bond length	0.006
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.610
Rmerge	0.038
Number of reflections	185277
Completeness [%]	87.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.2	290	protein concentration 8mg/ml, mother liquor 0.1M MgCl2, 15-18% PEG8000 and 0.1M Tris HCl (pH 9.2), VAPOR DIFFUSION, HANGING DROP, temperature 290K