4NB7
Crystal Structure of Two-Domain Laccase from Streptomyces LIvidans AC1709 in complex with azide after 180 min soaking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 177.510, 177.510, 177.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.377 - 2.550 |
R-factor | 0.162 |
Rwork | 0.161 |
R-free | 0.18110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gyb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.237 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 31563 | |
Completeness [%] | 99.5 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 296 | 31%(v/v) PEG monomethyl ether 550, 0.1M NaCl, 0.1M glycine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |