4NAV
Crystal structure of hypothetical protein XCC2798 from Xanthomonas campestris, Target EFI-508608
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-12 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.903, 156.017, 111.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.010 - 2.690 |
| R-factor | 0.24516 |
| Rwork | 0.244 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n1u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.510 | 2.830 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.083 | 0.426 |
| Number of reflections | 21866 | |
| <I/σ(I)> | 17.2 | 4.2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 273 | 85mM Tris-HCl, pH 8.5 0.17M sodium acetate 25.5% PEG4000,15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
