4NAD
Crystal Structure of the C-terminal Domain of CREPT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.280, 55.660, 84.450 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 42.171 - 2.800 |
R-factor | 0.2396 |
Rwork | 0.237 |
R-free | 0.28970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.565 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.171 | |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 14346 | |
Completeness [%] | 96.4 | 96.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 28% PEG 3350, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |