4NAA

Crystal structure of UVB photoreceptor UVR8 from Arabidopsis thaliana and UV-induced structural changes at 120K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-12
Detector	RAYONIX MX-300
Spacegroup name	C 1 2 1
Unit cell lengths	112.521, 77.336, 189.312
Unit cell angles	90.00, 95.62, 90.00

Refinement procedure

Resolution	31.090 - 1.670
R-factor	0.149
Rwork	0.147
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dnw
RMSD bond length	0.006
RMSD bond angle	1.046
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.670
Rmerge	0.039
Number of reflections	154616
<I/σ(I)>	23
Completeness [%]	82.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.2	290	protein 8mg/ml, 0.1M MgCl2 15-18% PEG8000 and 0.1M Tris HCl, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K