4N9W
Crystal structure of phosphatidyl mannosyltransferase PimA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0668 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.507, 75.515, 59.326 |
| Unit cell angles | 90.00, 97.69, 90.00 |
Refinement procedure
| Resolution | 21.688 - 1.940 |
| R-factor | 0.1642 |
| Rwork | 0.162 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.000 |
| High resolution limit [Å] | 1.940 |
| Rmerge | 0.065 |
| Number of reflections | 27870 |
| <I/σ(I)> | 9.3 |
| Completeness [%] | 98.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 16% PEG 8000, 200 mM MgCl2, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
