4N9V
High resolution x-ray structure of urate oxidase in complex with 8-azaxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.933 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.800, 95.080, 104.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.162 - 1.100 |
| R-factor | 0.1396 |
| Rwork | 0.139 |
| R-free | 0.15320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.049 | 0.413 |
| Number of reflections | 158812 | |
| <I/σ(I)> | 11.94 | 2.93 |
| Completeness [%] | 99.1 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 5 % PEG 8000, 0.1 M NACL, 0.1M TRISHCL PD 8.5, 8 MG/ML URATE OXIDASE, temperature-controlled batch, temperature 291K |
