4N9R
X-ray structure of the complex between hen egg white lysozyme and pentacholrocarbonyliridate(III) (1 day)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.319, 78.319, 37.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.450 - 1.550 |
R-factor | 0.184 |
Rwork | 0.157 |
R-free | 0.19420 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 193l |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 34873 |
Completeness [%] | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | Crystals of the complex have been obtained by soaking pre-grown HEWL crystals in a satured solution of Ir(Cl)5CO for 1 day, pH 4.5, VAPOR DIFFUSION, HANGING DROP |