4N9H
Crystal structure of Transcription regulation Protein CRP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 61 |
| Unit cell lengths | 120.617, 120.617, 60.349 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.20494 |
| Rwork | 0.202 |
| R-free | 0.26331 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.432 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.394 |
| Number of reflections | 25544 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 22.5 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 8000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
