4N97
E. coli sliding clamp in complex with 5-nitroindole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Detector technology | CCD |
| Collection date | 2011-08-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.880, 64.780, 72.590 |
| Unit cell angles | 73.91, 84.51, 84.89 |
Refinement procedure
| Resolution | 28.900 - 1.970 |
| R-factor | 0.2361 |
| Rwork | 0.232 |
| R-free | 0.30650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.554 | 28.901 | 2.080 |
| High resolution limit [Å] | 1.970 | 6.230 | 1.970 |
| Rmerge | 0.032 | 0.191 | |
| Total number of observations | 5232 | 20894 | |
| Number of reflections | 42650 | ||
| <I/σ(I)> | 12.7 | 15.4 | 2.2 |
| Completeness [%] | 84.8 | 94.1 | 85.2 |
| Redundancy | 3.6 | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
