4N8Z
In situ lysozyme crystallized on a MiTeGen micromesh with benzamidine ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.150, 79.150, 36.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.760 - 1.200 |
R-factor | 0.23007 |
Rwork | 0.229 |
R-free | 0.25073 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lyz |
RMSD bond length | 0.029 |
RMSD bond angle | 2.441 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.760 | |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.079 | 0.079 |
Number of reflections | 34003 | |
<I/σ(I)> | 30.46 | 30.46 |
Completeness [%] | 97.8 | 97.78 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | In situ crystallization on micromesh | 4.6 | 290 | 4% sodium chloride, 100mM sodium acetate pH 4.6, 120mg/ml, In situ crystallization on micromesh, temperature 290K |
1 | In situ crystallization on micromesh | 4.6 | 290 | 4% sodium chloride, 100mM sodium acetate pH 4.6, 120mg/ml, In situ crystallization on micromesh, temperature 290K |
1 | In situ crystallization on micromesh | 4.6 | 290 | 4% sodium chloride, 100mM sodium acetate pH 4.6, 120mg/ml, In situ crystallization on micromesh, temperature 290K |