4N8P

Crystal structure of a strand swapped CTLA-4 from Duckbill Platypus [PSI-NYSGRC-012711]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	I 21 21 21
Unit cell lengths	37.272, 107.991, 109.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.483 - 2.299
R-factor	0.1752
Rwork	0.173
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kkn
RMSD bond length	0.010
RMSD bond angle	1.318
Data reduction software	MOSFLM
Data scaling software	SCALA (0.1.29)
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	76.966
High resolution limit [Å]	2.299
Number of reflections	8805
Completeness [%]	85.3
Redundancy	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol), Reservoir (1.6M Ammonium sulfate, 0.1M MES, 10% (v/v) Dioxane), Cryoprotection (30% Glycerol), VAPOR DIFFUSION, SITTING DROP, temperature 298K