4N8L
E249D mutant, RipA structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 197.241, 197.241, 59.223 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.704 - 2.817 |
R-factor | 0.1843 |
Rwork | 0.182 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qlk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.975 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.147 | ||
Number of reflections | 32103 | ||
<I/σ(I)> | 18.02 | ||
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Bis Tris, 27% PEG monomethyl ether 2000, and 1mM propionyl-CoA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |