4N8K
E249A mutant, RipA structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.182, 108.176, 84.987 |
Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
Resolution | 43.585 - 2.000 |
R-factor | 0.1487 |
Rwork | 0.148 |
R-free | 0.17590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qlk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.159 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.071 | 0.033 | 0.442 |
Number of reflections | 62152 | ||
<I/σ(I)> | 9.6 | ||
Completeness [%] | 99.3 | 99.9 | 97 |
Redundancy | 3.8 | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M BisTris pH 5.5, 21% PEG 3350, 0.2M Ammonium sulfate, 1 mM succinyl CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |