4N8J
F60M mutant, RipA structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 197.307, 197.307, 59.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.286 - 2.010 |
| R-factor | 0.1404 |
| Rwork | 0.140 |
| R-free | 0.17370 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.082 | 0.059 | 0.396 |
| Number of reflections | 173491 | ||
| <I/σ(I)> | 17.5 | ||
| Completeness [%] | 97.9 | 99.9 | 79.5 |
| Redundancy | 5 | 5.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 0.4 M ammonium phosphate monobasic and 1 mM butyryl CoA, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
