4N8H
E61V mutant, RipA structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.907, 109.003, 85.014 |
| Unit cell angles | 90.00, 120.07, 90.00 |
Refinement procedure
| Resolution | 39.700 - 2.400 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qlk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.901 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.061 | 0.159 |
| Number of reflections | 36693 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 0.3 M Na malonate, 22% PEG 3350, and 1 mM propionyl CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
