4N8H
E61V mutant, RipA structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-19 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.907, 109.003, 85.014 |
Unit cell angles | 90.00, 120.07, 90.00 |
Refinement procedure
Resolution | 39.700 - 2.400 |
R-factor | 0.154 |
Rwork | 0.151 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qlk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.901 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.061 | 0.159 |
Number of reflections | 36693 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 298 | 0.3 M Na malonate, 22% PEG 3350, and 1 mM propionyl CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |