4N8F
CcmL from Thermosynechococcus elongatus BP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.901, 71.901, 197.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.18279 |
| Rwork | 0.180 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.778 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 35620 | |
| <I/σ(I)> | 19.7 | 2.6 |
| Completeness [%] | 99.1 | 91 |
| Redundancy | 6.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 273 | 1.8 M MgSO4, 100 mM MES, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
