4N7U
Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.980, 44.952, 125.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.220 - 1.460 |
| R-factor | 0.1552 |
| Rwork | 0.153 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.938 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.310 | 42.310 | 1.540 |
| High resolution limit [Å] | 1.460 | 4.620 | 1.460 |
| Number of reflections | 37549 | ||
| Completeness [%] | 96.3 | 82.8 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.2M MgCl2, 22%PEG3350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
