4N7G
Crystal structure of 14-3-3zeta in complex with a peptide derived from ExoS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 105.420, 105.420, 114.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.440 - 2.250 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.644 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.440 | 2.300 | |
| High resolution limit [Å] | 2.250 | 12.000 | 2.250 |
| Rmerge | 0.114 | 0.013 | 0.013 |
| Number of reflections | 35293 | 273 | 2203 |
| <I/σ(I)> | 19.69 | 39.97 | 4.46 |
| Completeness [%] | 100.0 | 96.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277.15 | 0.2 M sodium chloride, 0.1 M sodium acetate, pH 4.5, 40% v/v PEG300, VAPOR DIFFUSION, temperature 277.15K |
