4N7F

Crystal structure of 3rd WW domain of human Nedd4-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0000
Spacegroup name	P 41 2 2
Unit cell lengths	68.411, 68.411, 37.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.713 - 1.102
R-factor	0.1822
Rwork	0.181
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.700	1.490	1.140
High resolution limit [Å]	1.100	1.390	1.100
Number of reflections	36262
Completeness [%]	95.0	100	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	3.0M NaCl, 100mM Tris-HCl 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K