4N74
Crystal Structure of Outer Membrane Protein TamA beta-barrel Domain in E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-08-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.071, 71.071, 143.341 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.514 - 2.900 |
R-factor | 0.2368 |
Rwork | 0.232 |
R-free | 0.28130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.219 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((Phaser-MR: 1.8.1_1168)) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
Number of reflections | 48998 | ||
Completeness [%] | 99.0 | 95.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | 0.1M Glycine pH 9.0, 31% PEG 400, 0.15M magnesium chloride, 0.4M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 289K |