4N74

Crystal Structure of Outer Membrane Protein TamA beta-barrel Domain in E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-08-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	71.071, 71.071, 143.341
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.514 - 2.900
R-factor	0.2368
Rwork	0.232
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.219
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser-MR: 1.8.1_1168))
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Number of reflections	48998
Completeness [%]	99.0	95.8	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	0.1M Glycine pH 9.0, 31% PEG 400, 0.15M magnesium chloride, 0.4M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 289K