4N6T
Adhiron: a stable and versatile peptide display scaffold - full length adhiron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9686 |
Spacegroup name | P 41 |
Unit cell lengths | 35.855, 35.855, 61.847 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.855 - 1.750 |
R-factor | 0.1962 |
Rwork | 0.195 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.082 | 0.897 |
Number of reflections | 7889 | |
<I/σ(I)> | 18.9 | 2.6 |
Completeness [%] | 99.3 | 91.6 |
Redundancy | 12.8 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 280 | Morpheus (H05), 0.02 M Glycine, 0.02 M Sodium L-Glutamate, 0.02 M DL-Alanine, 0.02 M DL-Lysine Hydrochloride, 0.02 M DL-Serine, 0.1 M MOPS pH=7.50, 0.1 M HEPES sodium salt pH=7.50, 10.0% w/v Polyethylene glycol 20000, 20.0% w/v Polyethylene glycol 550 MME, VAPOR DIFFUSION, SITTING DROP, temperature 280K |