4N67
Crystal Structure of the N-terminal Fic Domain of a Putative Cell Filamentation protein (VirB-translocated Bep effector protein) with bound ADP from Bartonella quintana
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.450, 64.070, 97.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.550 |
| R-factor | 0.17326 |
| Rwork | 0.172 |
| R-free | 0.19419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.491 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 | |
| High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
| Rmerge | 0.046 | 0.026 | 0.490 |
| Number of reflections | 37518 | ||
| <I/σ(I)> | 22.71 | 52.52 | 3.51 |
| Completeness [%] | 99.6 | 90.7 | 99.3 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | JCSG+(g7): 01.M Succinic Acid, pH=7.0, 15% PEG-3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
