4N63
Crystal structure of hemagglutinin from an H7N9 influenza virus in complex with an O-linked glycan receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-06-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0331 |
Spacegroup name | P 21 3 |
Unit cell lengths | 153.608, 153.608, 153.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.603 - 2.752 |
R-factor | 0.2161 |
Rwork | 0.213 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n5j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.757 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.750 |
Rmerge | 0.095 |
Number of reflections | 31335 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.5 | 17-20% PEG3350, 0.2 M ammonium acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |