4N62

Crystal structure of hemagglutinin from an H7N9 influenza virus in complex with a sulfated receptor analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	110
Detector technology	CCD
Collection date	2013-06-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0331
Spacegroup name	P 21 3
Unit cell lengths	153.806, 153.806, 153.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.638 - 2.503
R-factor	0.2224
Rwork	0.220
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.853
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.089
Number of reflections	42019
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.5	17-20% PEG3350, 0.2 M ammonium acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K