4N5A

Crystal structure of Efr3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 64 2 2
Unit cell lengths	136.374, 136.374, 141.143
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.526 - 3.204
R-factor	0.2324
Rwork	0.231
R-free	0.25560
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.738
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	13197
Completeness [%]	99.4	100
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	1.35ul each of protein solution and mother liquor (0.08M sodium cacodylate, 0.16 M calcium acetate, 11.5% PEG 8000, 20% glycerol) and 0.3ul of 0.1 M EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K