4N53
Human enterovirus 71 uncoating intermediate captured at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 591.375, 591.375, 591.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.286 - 3.306 |
| R-factor | 0.2282 |
| Rwork | 0.228 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d4m |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.286 | 50.000 | 3.380 |
| High resolution limit [Å] | 3.306 | 8.130 | 3.306 |
| Rmerge | 0.216 | 0.143 | 0.701 |
| Number of reflections | 314499 | ||
| <I/σ(I)> | 4.4 | 9.74 | 0.93 |
| Completeness [%] | 62.2 | 80.8 | 46.2 |
| Redundancy | 1.5 | 2 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.5 | 289 | 0.1M Sodium Acetate containing 3.5M Sodium Formate, pH 4.5, hanging drop, temperature 289K |
