4N4Y

Structure of Recombinant Cytochrome ba3 Oxidase mutant G232V from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	144.150, 98.400, 114.100
Unit cell angles	90.00, 138.93, 90.00

Refinement procedure

Resolution	74.960 - 2.900
R-factor	0.17905
Rwork	0.177
R-free	0.22531
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.518
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.960
High resolution limit [Å]	2.900	2.900
Number of reflections	21798
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	50 mM sodium cacodylate, pH 6.5, 1.6 M sodium chloride, 40% PEG400, LIPIDIC CUBIC PHASE, temperature 293K