4N4K
Kuenenia stuttgartiensis hydroxylamine oxidoreductase soaked in hydroxylamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 130.070, 130.070, 130.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.990 - 2.200 |
R-factor | 0.16817 |
Rwork | 0.167 |
R-free | 0.18723 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4n4j |
RMSD bond length | 0.008 |
RMSD bond angle | 0.961 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.5.010) |
Refinement software | REFMAC (5.5.010) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.076 | 0.356 |
Number of reflections | 35645 | |
<I/σ(I)> | 17.3 | 4.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.3 M ammonium sulfate, 0.05-0.10 M sodium phosphate, pH 7.4, detergent additive cyclohexylbutanoyl-N-hydroxyethylglucamide (C-HEGA-10), VAPOR DIFFUSION, HANGING DROP, temperature 293K |