4N4G
Crystal structure of the Bromo-PWWP of the mouse zinc finger MYND-type containing 11 isoform alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.2825 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.919, 64.267, 75.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.134 - 1.947 |
R-factor | 0.1899 |
Rwork | 0.188 |
R-free | 0.22630 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.924 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.947 | 5.290 | 1.947 |
Rmerge | 0.081 | 0.040 | 0.786 |
Number of reflections | 37810 | ||
<I/σ(I)> | 19.7 | ||
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 6.3 | 6.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 8% (w/v) polyethylene glycol 3000, 0.1M Imidazole, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |