4N4B
Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.410, 66.830, 60.570 |
| Unit cell angles | 90.00, 117.39, 90.00 |
Refinement procedure
| Resolution | 44.610 - 1.440 |
| R-factor | 0.12931 |
| Rwork | 0.128 |
| R-free | 0.15192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n1i |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.521 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.045 | |
| Number of reflections | 60688 | |
| <I/σ(I)> | 21.86 | 8.26 |
| Completeness [%] | 92.3 | 80.3 |
| Redundancy | 4.56 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | 25% PEG 4000, 0.2M Calcium chloride 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
