4N4A

Cystal structure of Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-12
Detector	RAYONIX MX-225
Wavelength(s)	0.918410
Spacegroup name	I 4 2 2
Unit cell lengths	139.359, 139.359, 146.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.745 - 2.350
R-factor	0.156
Rwork	0.154
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.154
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	30223
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	35% Tacsimate , pH 7.0, VAPOR DIFFUSION, temperature 291K