4N4A
Cystal structure of Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-12 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.918410 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 139.359, 139.359, 146.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.745 - 2.350 |
R-factor | 0.156 |
Rwork | 0.154 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.350 |
Number of reflections | 30223 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 35% Tacsimate , pH 7.0, VAPOR DIFFUSION, temperature 291K |