4N42
Crystal structure of allergen protein scam1 from Scadoxus multiflorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.736, 65.265, 49.320 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 65.200 - 2.200 |
| R-factor | 0.15545 |
| Rwork | 0.153 |
| R-free | 0.19618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o9n |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.260 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 15078 |
| <I/σ(I)> | 7.8 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1M sodium acetate, 20% PEG 6000, VAPOR DIFFUSION, HANGING DROP, pH 7.2, temperature 298K |
