4N40
Crystal structure of human Epithelial cell-transforming sequence 2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 |
| Unit cell lengths | 123.700, 123.700, 89.244 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.873 - 3.106 |
| R-factor | 0.2169 |
| Rwork | 0.214 |
| R-free | 0.28140 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.098 | 0.072 | 0.956 |
| Number of reflections | 9161 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 99.9 | 97.8 | 99.8 |
| Redundancy | 4.4 | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 100mM MES, 14% MPEG 5000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 100mM MES, 14% MPEG 5000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
