4N3O
2.4 Angstrom Resolution Crystal Structure of Putative Sugar Kinase from Campylobacter jejuni.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.609, 85.796, 77.947 |
| Unit cell angles | 90.00, 122.39, 90.00 |
Refinement procedure
| Resolution | 29.160 - 2.400 |
| R-factor | 0.17973 |
| Rwork | 0.177 |
| R-free | 0.23229 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.577 |
| Number of reflections | 26058 | |
| <I/σ(I)> | 40.7 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | Protein: 1.5mg/mL, 0.3M Sodium cloride, 0.1M HEPES pH 7.5; Screen: 0.2M Calcium acetate, 0.1M Na Cacodylate pH 6.0, 18% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
